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Image Search Results
Journal: Journal of Clinical Medicine
Article Title: Bicalutamide Exhibits Potential to Damage Kidney via Destroying Complex I and Affecting Mitochondrial Dynamics
doi: 10.3390/jcm11010135
Figure Lengend Snippet: Representative western blot and densitometric analysis showing level of PGC1α ( a ), MFN1 ( b ), MFN2 ( c ), OPA1 long form (L-OPA1, 95kD) and short form (S-OPA1, 75kD) ( d ), and DRP1 ( e ) proteins in RMC cells following 0, 30, and 60 μM of bicalutamide treatment for 24 and 48 h. β-Actin is as cytosolic normalization control; HSP60 is as mitochondrial normalization control. Bar graph represents the mean ± SD of triplicate experiments: * p < 0.05, ** p < 0.01 *** p < 0.001 compared to Bic 0 μM at 24 h. ## p < 0.01 compared to Bic 30 μM at 24 h. § p < 0.05, §§ p < 0.01, §§§ p < 0.001 compared to Bic 0 μM at 48 h. p < 0.001 compared to Bic 30 μM at 48 h.
Article Snippet: The membranes were then incubated for 16 h at 4 °C with anti-Tomm 20 (GTX133756), anti-NOX4 (GTX121929) and anti-Mitofusin 1 (MFN1) (GTX133351) (GeneTex, Inc., Irvine, CA, USA), anti-NDUFB8 (tccua 57905), anti-SIRT1 (tcea19648), anti-SIRT3 (tcna3378),
Techniques: Western Blot